Target Discovery of Flavonoids from Elymus nutans Griseb Using Medium- and High-Pressure Liquid Chromatography Combined with Online High-Performance Liquid Chromatography–1,1-diphenyl-2-picrylhydrazyl Detection

Forage-based nutrients constitute the main forage value of grass. Elymus nutans Griseb possesses a wide ecological adaptability, enhanced crude protein content, good palatability, and excellent genes. Herein, employing medium- (MPLC) high-pressure liquid chromatography (HPLC), along with online HPLC-DPPH (OHD)-based identification, two primary radical scavenging compounds were extracted identified from methanolic extract Griseb. With starting material 300 g Griseb, 5.95 target DPPH suppressors fraction (Fr6) was separated following one cycle MCI GEL® CHP20P medium pressure chromatography. A Kromasil 100-5-Phenyl column subsequently employed for further purification fraction, which yielded 432.16 mg Fr62 (7.26% recovery) 489.01 Fr63 (8.22% recovery). The then assessed based on their structure purity, (salcolin tricin) > 95% purity. This newly designed procedure highly effective targeted flavonoids, high-purity scavenger extraction extracts. methodology can potentially provide scientific basis quality evaluation.